Identification of animal derivatives contained in commercial chicken feeds using multiplex-PCR

Detection of carbapenemases in Enterobacteriaceae by a commercial multiplex PCR.

A commercial multiplex PCR (hyplex SuperBug ID) was tested with a collection of 132 clinical Enterobacteriaceae strains producing different carbapenemases. The sensitivity for the detection of KPC-, VIM-, NDM-, and OXA-48-encoding genes was 100%, whereas two IMP variants were missed.

Molecular identification of agrobacterium tumefaciens containing pCAMBIA 1305.2 plasmid using multiplex PCR and Gold nanoparticles multiplex probe

Conventional microbiology methods used to detect bacteria include multiple cultures and identification processes, so the results of lab work are painstaking and time-consuming. In recent years, more and more tend to use the diagnostic tests which are based on DNA; hence, DNA diagnostic biosensors have been created to perform DNA identification better. In this study, GUS and hpt genes were used ...

Investigation of Aflatoxins in Imported Animal Feeds in Iran

Background: Aflatoxins (AF’s) are secondary metabolites produced by certain species of fungi on grains and animal feeds. Because of serious impact of AF’s on health, they should be monitored closely in potentially hazardous foods and feeds. Method: In this study, AF’s in imported animal feeds including cereals, corn gluten and meals (pastes derived from the cotton, colza and sunflower seeds) f...

Messenger Rna Profiling: Body Identification Using Multiplex Real-time Pcr

Conventional methods of body fluid identification use labor-intensive, technologically diverse techniques that are performed in a series, not parallel, manner and are costly in terms of time and sample. Furthermore, for some frequently encountered body fluids no confirmatory technique exists. There is no definitive test, for example, for the presence of saliva or vaginal secretions. In seeking ...

mRNA Profiling: Body Fluid Identification Using Multiplex Real-Time PCR